What is ATP Testing - Cleaning Verification

What is ATP Cleaning Verification

ATP is a Cleaning Verification Systems to help organizations achieve optimal standardized cleaning levels. These systems use bioluminescence technology to identify and measure adenosine triphosphate, commonly known as ATP.

This measurement helps organizations objectively determine from a microbiological level if a surface has been cleaned properly and is safe for use, or if it requires a corrective action such as re-cleaning.

- What is ATP? ATP is an energy molecule found in all living cells that allows cellular metabolism to take place. All organic matter contains ATP. In healthcare facilities, organic matter such as bodily fluids, blood, and bacteria left on surfaces can become a point of cross contamination between patients and staff, which can lead to infections if not properly cleaned. Therefore, the detection of ATP on a surface after cleaning is an indication of cleaning efficacy.

What is bioluminescence? ATP tests contain an enzyme called luciferase which produces a bioluminescence (light-producing) reaction when it comes into contact with ATP. The light emitted from the reaction is measured and quantified in a luminometer. The unit of measurement for the emitted light is Relative Light Units (RLU).

- Higher Contamination = Higher RLU - The quantity of light generated by the bioluminescence reaction is directly proportional to the amount of ATP present in the sample. The reaction is immediate, allowing results to be processed in real-time. Results are expressed numerically on the screen in RLU.


Step 3: Performing a Test – 
Running an ATP Test on a ATP Cleaning Verification System can be done by following these steps:

1. Power on your luminometer and grab the desired amount of ATP Test swabs.

2. Sample Collection – remove the swab from the tube and swab a 4 x 4 inch area on the desired test surface. Then replace the swab back into the tube.

3. Sample Activation – Break the Snap-Valve at the top of the test by bending it forward and backwards and then squeeze the bulb to expel the liquid into the tube. Shake the tube for 5 seconds to allow the liquid to come in contact with the swab tip.

4. Measurement – If you have inputted plans and locations, select the desired location. Insert the swab into the read chamber and close the lid. Hold the luminometer upright, and press “OK” to initiate the test. Once the on-screen count-down has completed, your test results will be shown on the screen.